Next-Generation Live Cell Assays with Livecyte (Phasefocus)

15:00 – 15:05 GMT, 6 January 2021 ‐ 5 mins

Session Four

Presenter: Dr. Martin Humphry

In this talk we will briefly show, via a software demo, how easy it is to gain powerful insights into cell behaviour and drug responses in live-cell assays using a Livecyte Kinetic Cytometer.

Livecyte is the only commercial instrument optimised for high-content long-term live cell assays with automated cell tracking.  Label-free imaging, with extremely low power illumination means that even the most sensitive cells can be imaged for days or weeks at time without adverse effects. With Phasefocus’s patented Ptychographic quantitative phase imaging technology at its core, Livecyte can produce high contrast “fluorescence-like” images, without the phototoxic effects. These images are ideally suited to automated segmentation and single cell tracking, enabling the morphology and motion of statistically significant numbers of cells to be monitored over time, in up to 96 well plate format. Powerful, integrated analysis software helps you to make sense of such deep data, from the assay-level Dashboards which give you an overview of different types of phenotypic behaviour in your populations at the click of a button, to deep-diving right down to individuals within those populations and how they behave. We’ll show just how easy this is with a software demonstration.

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Figure 1 - A Livecyte Kinetic Cytometer

 

 

 

Make faster decisions, and quickly uncover phenotypic differences, with graphical proliferation, morphology, scratch-wound and motility dashboards, available immediately after each experiment. Add in correlative fluorescence measurements (up to 7 channels) and that makes Livecyte the most comprehensive live cell analysis instrument on the market. Find out more at Phasefocus.com/livecyte.

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Figure 2 - Livecyte's Random Motility Dashboard