Challenges and Perspectives of in toto and in vivo Small Animal Imaging with Synchrotron Radiation

14:30 – 14:50 BST, 17 July 2024 ‐ 20 mins

Tilo Bamubach

The talk will discuss recent results and perspectives for synchrotron based 2D/3D/4D full-field hard X-ray imaging techniques at KIT with a special focus on challenges of in toto up to in vivo small animal imaging with synchrotron radiation. In particular, current developments, new approaches and further challenges towards (I) dose-efficient propagation-based phase-contrast imaging, (II) hierarchical imaging of large, flat samples and (III) serial computed microtomography will be outlined.
This will be illustrated by first results and future plans related to the new KIT experimental stations LAMINO-II and UFO-II, currently under development at the superconducting wiggler beamline IMAGE at the KIT Light Source, and KIT´s new HIKA station for hierarchical imaging and serial tomography at the P23 undulator beamline at PETRA III, DESY. The talk shows their methodical capabilities and gives application examples of small animal studies, in particular, (1) dose-efficient  micrometer-resolved imaging close to the theoretical limit by using coherent asymmetric crystal optics, enabling, e.g., unprecedented in vivo behavioral studies of parasitoid insects within their hosts, (2) drastic contrast enhancement for low resolution propagation-based imaging of soft tissues by realizing kilometer-long propagation distances, (3) hierarchical 3D imaging by 3D screening of large areas and zooming into selected regions of interest using computed laminography guided by on-the-fly data processing, e.g., for paleontological studies of compression fossils, (4) large comparative 3D morphological studies of thousands of specimen, such as insects or vertebrate model organisms for correlation with genetic and environmental meta data.