This two day annual confocal course utilises many different sample types and fluorescent probes (DNA stains, classic antibody labels and fluorescent proteins) which are chosen to best demonstrate particular problems and techniques. Focus is always on the techniques they enable and the problems they generate, which will be applicable to any sample types.
Day 1 takes participants through the basic principles of confocal microscopy and then shows them how to configure and image multicolour, multidimensional samples using a confocal microscope.
Day 2 builds on the experience of day 1 and demonstrates FRAP and spectral profiling.
Scientific Organiser RMS Organiser
Peter O'Toole
Peter heads the Imaging and Cytometry Labs within the Technology Facility at the University of York which includes an array of confocal microscopes, flow cytometers and electron microscopes. Peter gained his PhD in the Cell Biophysics Laboratory at the University of Essex and has been involved in many aspects of fluorescence imaging. Research is currently focused on both technology and method development of novel probes and imaging modalities.
Peter has ongoing collaborations with many leading microscopy and cytometry companies and his group also provides research support to many academics and commercial organisations. Peter is also heavily involved with teaching microscopy and flow cytometry which includes organising and teaching on both the RMS Light Microscopy Summer School and the RMS Practical Flow Cytometry courses.
Debbie Hunt
debbie@rms.org.uk
+44(0)1865 254766
Contact Debbie for RMS membership enquires, Summer Studentships, Bursaries, RMS Courses, History Committee queries and microscope donations.