All timings are British Summer Time (BST)

Please note this programme is provisional and may change.

Wednesday 11 June
09:15 – 09:30
Registration

Administration

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09:30 – 10:30
Basics of Confocal Microscopy (Fluorescence/Filters)

Lecture

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10:30 – 11:00
Tea/Coffee

Break

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11:00 – 12:45
Basics of Setting up (Laser Power versus Voltage Gain, Dichroic & Emission Filters, Z-Sections, Averaging, Image Size, Comparing Methods Simultaneous versus sequential)

Practical

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12:45 – 13:30
Lunch

Break

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13:30 – 15:00
Basics of Multicolour Images

Practical

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15:00 – 15:30
Tea/Coffee

Break

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15:30 – 17:00
Z-Stacks & Tiling with Reflection

Practical

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Thursday 12 June
09:30 – 10:30
Live Cell Imaging and FRAP (Tips for Live Cell Work, Different Techniques Available, FRAP Description, Pros and Cons) & Spectal Imaging

Lecture

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10:30 – 11:00
Tea/Coffee

Break

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11:00 – 12:30
Live Cell Imaging with FRAP (Time and Depth of Bleach, Effective Laser Spot Size, Slow FRAP)

Practical

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12:30 – 13:15
Lunch

Break

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13:15 – 14:30
Live Cell Imaging with FRAP (Time and Depth of Bleach, Effective Laser Spot Size, Fast FRAP)

Practical

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14:30 – 15:00
Spectral Unmixing

Practical

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15:00 – 15:30
Tea/Coffee

Break

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15:30 – 17:00
Spectral Unmixing

Practical

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