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- Flow Cytometry Course 2025
- Provisional Programme
All timings are British Summer Time (BST)
Please note this programme is provisional and may change.
Monday 8 September
13:30 – 14:30
The Flow Cytometers (Including Routine Checks of Performance – Amplifier Linearity, Sensitivity)
Demonstration
14:30 – 15:00
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour beads)
Demonstration
Tuesday 9 September
09:15 – 10:45
Multiparameter Labelling – Fluorescence Compensation (Peripheral Blood Lymphocytes – Colour Compensation)
Demonstration
11:15 – 13:00
Multiparameter Labelling – Fluorescence Compensation (Peripheral Blood Lymphocytes – Colour Compensation)
Demonstration
13:45 – 15:15
Linear Analysis & Doublet Discrimination - Using Cell Cycle as Examples
Demonstration
Wednesday 10 September
09:15 – 10:00
Analysis of Micro-Organisms & Sub-Micron Particles
Lecture (Research Stream)
14:00 – 14:45
Intracellular Staining (including fixation & permeabilisation)
Lecture (Research Stream)
16:15 – 17:30
Multicolour Cytometry, Compensation and Experimental Planning
Lecture (Research Stream)
Multicolour Cytometry, Compensation and Experimental Planning
Lecture (Clinical Stream)
Thursday 11 September
CD4 and Immunodeficiency
Lecture (Clinical Stream)
14:15 – 15:15
Apoptosis: a) Live/dead, b) Sub-G1 Peak, c) Annexin V labelling, d) Dye uptake, e) Mitochondrial Membrane Potential
Demonstration
Probes/Stains/Reagents
Lecture (Clinical Stream)