All timings are British Summer Time (BST)

Please note this programme is provisional and may change.

Wednesday 10 July
09:15 – 09:30
Registration

Administration

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09:30 – 10:30
Basics of Confocal Microscopy

Lecture

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10:30 – 11:00
Tea/Coffee

Break

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11:00 – 13:00
Basics of Setting up Basic and Multicolour Images (Laser Power versus Voltage Gain, Dichroic and Emission Filters, Z-Sections, Averaging, Image Size, Comparing Methods Simultaneous versus Sequential)

Practical

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13:00 – 14:00
Lunch

Break

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14:00 – 15:30
Basics of Setting up Basic and Multicolour Images

Practical

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15:30 – 16:00
Tea/Coffee

Break

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16:00 – 17:00
Reflection with Confocal Microscopes

Lecture

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Thursday 11 July
09:30 – 10:30
Live Cell Imaging and FRAP (Tips for Live Cell Work, Different Techniques Available, FRAP Description, Pros and Cons) & Spectral Imaging

Lecture

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10:30 – 11:00
Tea/Coffee

Break

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11:00 – 12:30
Live Cell Imaging with FRAP (Time and Depth of Bleach, Effective Laser Spot Size, Slow FRAP)

Practical

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12:30 – 13:15
Lunch

Break

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13:15 – 14:30
Live Cell Imaging with FRAP (Time and Depth of Bleach, Effective Laser Spot Size, Fast FRAP)

Practical

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14:30 – 15:00
Spectral Unmixing

Practical

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15:00 – 15:30
Tea/Coffee

Break

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15:30 – 17:00
Spectral Unmixing

Practical

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