All timings are British Summer Time (BST)

Please note this programme is provisional and may change.

Clinical Stream
Monday 2 September
09:00 – 09:30
Registration

Administration

09:30 – 10:00
Fluorochromes and Fluorescence

Lecture

10:00 – 10:25
Antibody Labelling and Immunofluorescence

Lecture

10:25 – 10:55
Tea/Coffee

Break

10:55 – 11:40
Introduction to the Flow Cytometer: Fluidics, Optics and Lasers

Lecture

11:40 – 12:30
Detectors and Signal Processing

Lecture

12:30 – 12:50
System Walk-Around

Practical

12:50 – 13:30
Lunch

Break

13:30 – 14:30
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)

Practical

14:30 – 15:00
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)

Practical

15:00 – 15:30
Tea/Coffee

Break

15:30 – 16:30
Multicolour Design and Compensation

Lecture

16:30 – 17:20
Executing a Flow Cytometry Experiment

Lecture

Tuesday 3 September
09:15 – 10:45
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)

Practical

10:45 – 11:15
Break

Break

11:15 – 13:00
Practical Continued

Practical

13:00 – 13:45
Lunch

Break

13:45 – 15:15
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples

Practical

15:15 – 16:00
Tea/Coffee and Questions: Optional session for one-to-one or group questions

Break

Wednesday 4 September
09:00 – 09:15
Registration for Advanced Course Attendees

Administration

09:15 – 10:15
Clinical Assay Design, Guidelines and Best Practice

Lecture

10:15 – 10:45
Integrated Reporting

Lecture

10:45 – 11:15
Tea/Coffee and Trade Exhibition

Break

11:15 – 11:45
Lymphoproliferative Disease

Lecture

11:45 – 12:15
Case Studies

Lecture

12:15 – 12:45
CD34

Lecture

12:45 – 13:45
Lunch and Trade Exhibition

Break

13:45 – 15:00
PNH/FMH

Lecture

15:00 – 15:45
CD34 Enumeration

Practical

15:45 – 16:15
Tea/Coffee and Trade Exhibition

Break

16:15 – 17:30
Multicolour Cytometry, Compensation and Experimental Planning

Lecture

Thursday 5 September
09:15 – 10:00
Big Data

Lecture

10:00 – 10:45
CD4 and Immunodeficiency

Lecture

10:45 – 11:15
Tea/Coffee

Break

11:15 – 12:00
Acute Lymphoblastic Leukaemia

Lecture

12:00 – 12:45
Myeloid Disorders

Lecture

12:45 – 13:45
Lunch

Break

13:45 – 14:45
Case Studies

Lecture

14:45 – 15:15
Myeloma/Plasma Cells

Discussion

15:15 – 15:45
Tea/Coffee

Break

15:45 – 16:15
Rare Event Analysis

Lecture

16:15 – 16:45
Rare Event Analysis

Practical

16:45 – 17:15
Probes/Stains/Reagents

Lecture

Friday 6 September
09:30 – 11:00
Quality Assurance, Data Analysis & Reporting

Workshop

11:00 – 11:30
Tea/Coffee

Break

11:30 – 12:15
Spectral Flow Cytometry

Lecture

12:15 – 12:45
Data Presentation Guidelines

Lecture

12:45 – 13:30
Lunch

Break

13:30 – 14:00
Other Applications of Flow Cytometry

Lecture

14:00 – 15:00
New Technologies in Imaging and Cytometry

Lecture

 

Research Stream
Monday 2 September
09:00 – 09:30
Registration

Administration

09:30 – 10:00
Fluorochromes and Fluorescence

Lecture

10:00 – 10:25
Antibody Labelling and Immunofluorescence

Lecture

10:25 – 10:55
Tea/Coffee

Break

10:55 – 11:40
Introduction to the Flow Cytometer: Fluidics, Optics and Lasers

Lecture

11:40 – 12:30
Detectors and Signal Processing

Lecture

12:30 – 12:50
System Walk-Around

Practical

12:50 – 13:30
Lunch

Break

13:30 – 14:30
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)

Practical

14:30 – 15:00
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)

Practical

15:00 – 15:30
Tea/Coffee

Break

15:30 – 16:30
Multicolour Design and Compensation

Lecture

16:30 – 17:20
Executing a Flow Cytometry Experiment

Lecture

Tuesday 3 September
09:15 – 10:45
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)

Practical

10:45 – 11:15
Break

Break

11:15 – 13:00
Practical Continued

Practical

13:00 – 13:45
Lunch

Break

13:45 – 15:15
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples

Practical

15:15 – 16:00
Tea/Coffee and Questions: Optional session for one-to-one or group questions

Break

Wednesday 4 September
09:00 – 09:15
Registration for Advanced Course Attendees

Administration

09:15 – 10:00
Analysis of Micro-Organisms and Sub-micron Particles

Lecture

10:00 – 10:45
Receptor Quantification

Lecture

10:45 – 11:15
Tea/Coffee and Trade Exhibition

Break

11:15 – 12:00
Receptor Quantification

Practical

12:00 – 13:00
Studying Bacteria

Practical

13:00 – 14:00
Lunch and Trade Exhibition

Break

14:00 – 14:45
Intracellular Staining (including fixation and permeabilisation)

Lecture

14:45 – 15:45
Proliferation

Lecture

15:45 – 16:15
Tea/Coffee and Trade Exhibition

Break

16:15 – 17:30
Multicolour Cytometry, Compensation and Experimental Planning

Lecture

Thursday 5 September
09:15 – 10:00
Big Data

Lecture

10:00 – 10:45
Studying Apoptosis by Flow Cytometry

Lecture

10:45 – 11:15
Tea/Coffee

Break

11:15 – 11:45
Measurement of Cell Proliferation: The Dye Dilution Method

Lecture

11:45 – 12:15
Measurement of Cell Cycle Parameters using BrdUrd/Anti BrUrd

Practical

12:15 – 12:45
Cell Proliferation by Dye Dilution

Practical

12:45 – 13:45
Lunch

Break

13:45 – 14:15
Antibody Titration

Practical

14:15 – 15:15
Apoptosis: a/ Live/Dead, b) Sub-G1 Peak, c) Annexin V Labelling, d) Dye Uptake, e) Mitochondrial Membrane Potential

Practical

15:15 – 15:45
Tea/Coffee

Break

15:45 – 16:45
Fluorescent Proteins, FRET and Flow Cytometry

Lecture

16:45 – 17:15
Probes/Stains/Reagents

Lecture

Friday 6 September
09:30 – 10:15
Imaging Flow Cytometry

Lecture

10:15 – 11:00
High Speed Cell Sorting

Practical

11:00 – 11:30
Tea/Coffee

Break

11:30 – 12:15
Spectral Flow Cytometry

Lecture

12:15 – 12:45
Data Presentation Guidelines

Lecture

12:45 – 13:30
Lunch

Break

13:30 – 14:00
Other Applications of Flow Cytometry

Lecture

14:00 – 15:00
New Technologies in Imaging and Cytometry

Lecture