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- Flow Cytometry Course 2023
- Provisional Programme
Clinical Stream
Monday 4 September
13:30 – 14:30
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)
Practical
14:30 – 15:00
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)
Practical
Tuesday 5 September
09:15 – 10:45
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)
Practical
13:45 – 15:15
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples
Practical
15:15 – 16:00
Tea/Coffee and Questions: Optional session for one-to-one or group questions
Break
Research Stream
Monday 4 September
13:30 – 14:30
The Flow Cytometers (including routine checks of performance - amplifier linearity, sensitivity)
Practical
14:30 – 15:00
Quality Control in Flow Cytometry using Commercially Available Beads (8-peak beads and other multipeak/colour)
Practical
Tuesday 5 September
09:15 – 10:45
Multiparamater Labelling - Fluorescence Compensation (peripheral blood lymphocytes - colour compensation)
Practical
13:45 – 15:15
Linear Analysis and Doublet Discrimination - Using Cell Cycle as Examples
Practical
15:15 – 16:00
Tea/Coffee and Questions: Optional session for one-to-one or group questions
Break
Thursday 7 September
14:15 – 15:15
Apoptosis: a/ Live/Dead, b) Sub-G1 Peak, c) Annexin V Labelling, d) Dye Uptake, e) Mitochondrial Membrane Potential
Practical