infocus #65 March 2022 2021 Summer Studentship Report: Localisation and distribution of the activity regulated cytoskeleton associated protein (Arc) in the mammalian brain

During this internship, I learnt important techniques in sample preparation of brain tissue for microscopy and the use of widefield as well as confocal microscopes to visualise the anatomical distribution of proteins in the brain.

DOI: 10.22443/rms.inf.1.224

Using these two different microscopes, I was able to get a glimpse of a fascinating hidden world. I enjoyed developing and re-assessing workflows to achieve the best visualisation of different brain regions. These workflows ranged from sample preparation to the settings of the microscope. Often minute changes in the workflow affected the experiment and, therefore, needed to be re-evaluated and, if necessary, adapted. Additionally, I learnt to process images taken on the cryogenic electron microscope (cryoEM).

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