General Microscopy

Escherichia coli has been chosen as a highly promising and robust cell factory for heterologous production of nonribosomal peptides and polyketides, given its versatile advantages including easy cultivation, genetic manipulation and scalable fermentation. Heterologous production of natural products in Escherichia coli has emerged as an attractive strategy to obtain molecules of interest. Laboratory scale production is technically feasible. For enzyme production, the nature of the production host organism and selection of plasmid vector are crucial to the expression of a particular enzyme. Genetic manipulation for the production of heterologous proteins in E. coli has been progressively improved. Achieving a fast growth rate up to one generation per twenty minutes, under optimized conditions makes it an attractive procedure. As compared to other hosts, competent cells preparation is relatively easier and transformation of foreign plasmid DNA in E. coli is eminently successful and efficient.

Created: 27 Dec 2024 07:12:41 AM