Antibody titrations on spectral instruments - do you use raw data or unmixed data for analysis and why?
Hi all. To determine the SI of my antibody-fluorophore, I have always unmixed titration data on our Cytek Auroras (even if only using one colour) before analysis. This tends to give me better resolution as it's removing cell autofluorescence and is using all the instrument data rather than just one detector. I noticed recently that Cytek has a 'how to' sheet for titration and they use the raw data - using the primary channel.
Question - what do you (the experts use) and why? Is raw data better to use than unmixed?
Created: 04 Nov 2024 10:11:46 AM
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