University of Vermont Microscopy Imaging Center

The Microscopy Imaging Center (MIC) is a resource for educating and assisting investigators in all aspects of sample preparation and collection and analysis of images for biological and materials applications.. It is operated on a fee for service basis and provides professional consultation and assistance with experimental design, equipment use, and interpretation and analysis of results.



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Imaging Platforms 

Keywords - Light Microscopy: Cryo-fluorescence, Superresolution microscopy, Laser microdissection, Live cell imaging, Atomic Force Microscopy, Confocal microscopy, Widefield microscopy, TIRF, Multi-photon microscopy, STORM
Keywords - Electron Microscopy: Transmission EM, Scanning EM (Tungsten), Cryo-TEM 

JEOL 1400 TEM, JEOL 6060 SEM with attached Oxford INCA EDS system, FEI Tecnai T12 cryoelectron microscope, Nikon N-STORM super-resolution microscope, Nikon A1R HD confocal scanning laser microscope with spectral detector and high-throughput imaging, Nikon C2 confocal scanning laser microscope, Yokogawa CSU-W1 spinning disk confocal system, Zeiss LSM-7 MP multiphoton confocal microscope, Akoya CODEX multiplex immunostaining system, Olympus BX50 widefield fluorescence microscope and IX70 inverted microscope, Arcturus XT-TI laser capture microdissector, Asylum Instruments MFP-3D-BIO and Cypher ES atomic force microscopes, Leica-Aperio VERSA-8 whole slide imager, Caliper Life Sciences IVIS Lumina whole animal imaging system, dedicated workstations for image analysis and processing, including NIS Elements, MetaMorph, Volocity, HALO, and Stereo Investigator., and a full histology suite, including a Lecia Bond RMx autostainer and RNAScope. 

 

Applications

Keywords - Biological: Geology/Mineralogy, Nanomaterials
Keywords - Physical Sciences: Resin embedding, Serial sectioning, Cryosectioning, Plunge freezing, Critical point drying, Metal coating, Immunofluorescence, Enzyme histochemistry, Ultrathin sectioning, Immunolabelling, Negative stain, Cryostat sectioning, Live cell

 

Data Analysis 

Keywords - Software: Volocity, Metamorph

Nikon NIS Elements; Indica Labs HALO 



Point of Contact

University of Vermont Microscopy Imaging Center
Health Sciences Research Facility 203
Microscopy Imaging Center, University of Vermont
Burlington, VT, 05405, United States


Point of Contact

Douglas J. Taatjes, PhD

 

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