Expertise
Platforms
KEYWORDS - PLATFORMS: COnventional FLOW CYTOMETRY. Imaging Flow CYtometry. Full spectrum flow cytometry. CELL SORTING.
The facility has 2 CytoFLEX (Beckman Coulter) conventional flow cytometers, an ID7000 (SONY) full spectrum flow cytometer. Imaging flow cytometry is provided by an ImageStream X MKII (Luminex). Full training is provided for use of these analytical machines. High speed cell sorting is offered as a service on the Astrios EQ (Beckman Coulter).
Applications
Keywords - Applications: Phenotyping | Cell cycle | Mammalian cell | Plant biology | Yeast Biology | Bacteria | Fluorescent proteins | Clinical studies
The facility has expertise in all areas of flow cytometry and cell sorting including bacteria and small particle analysis.
Data Analysis
KEYWORDS - SOFTWARE: KALUZA | Flow JO | FCS Express | IDEAS | unsupervised analysis
The Facility runs monthly training courses in Kaluza software and also supports users with FlowJo (including R plugins for unsupervised analysis), FCS express, and IDEAS.
Shared Access
The Facility is open to all users in the University of Nottingham and by arrangement to external parties. We have an online booking system to facilitate open use