Expertise
Imaging Platforms
Keywords - Light Microscopy: Confocal microscopy | Widefield microscopy | Live cell imaging | Multi-photon microscopy | Core facility | Slide scanning | Ptychography | Digital Holography | Microscopy courses | Label Free Imaging | Super-resolution Airyscan | High Plex Imaging | LSCM | Slide Scanning | QPI | Tissue Imaging | FRAP, FRET, FRET, FCS, FCCS, TIRF, PALM, STORM | SIM spectral imaging | high content screening | Multiphoton | QPM | live-cell imaging | 96 well plate imaging | Laser-trapping, optical tweezers and LSCM
Keywords - Electron Microscopy: Transmission EM | Scanning Transmission EM | Scanning EM (FEG) | Cryo-SEM | Probe development and high resolution imaging | TEM | SEM | cryo-EM | Cryo-TEM | Low pressure SEM | liquid EM | Volume EM | vEM | Elemental Analysis | EDAX
Keywords: Flow Cytometry | FACS | Cell Sorting | single cell deposition | single cell analysis | sterile sorting | Spectral Cytometry | Imaging Cytometry | Imaging Cell Sorting
The facility houses:
Light microscopy
- Laser Scanning Confocal Microscopes - 3 Zeiss laser scanning microscopes; 2x Zeiss LSM980 (one on an invert with Airyscan 2 and multiphoton, one on an upright stand) LSM880 with AiryScan. All equipped with a range of lasers (405-633nm), objectives and live-cell incubators
- Zeiss ELYRA 7 for SIM/PALM/STORM and live cell imaging
- Cytation 5 for 96 well plate fluorescence imaging with live cell incubation
- Zeiss Axio Observer 7 for fluorescence and brightfield imaging including the Colibri7 and Definite Focus with live cell incubation
- Slide Scanner - Zeiss AxioScan Z1 fluorescence and brightfield slide scanner
- Live-Cell Imaging - The Phase Focus Livecyte 2 (label-free ptychographic Quantitative Phase Imaging system) and Tomocube systems for high-resolution 3D digital holography imaging. All with live cell and fluorescence and multiwell plate options
- Tissue Imaging – Nanostring DSP for up to 80 simultaneous antibody analysis and 15,000 RNA analysis and 10X Visium options catering for most spatial omics approaches
Electron Microscopy
- Zeiss Gemini 460 FEGSEM - 200V to 30kV with Off-axis SE2, In-Lens SE1, In-Lens ESB, Volume-BSD, a-STEM, Octane Elite EDX detectors. Including the VoluTome diamond knife in-chamber ultramicrotome for serial block face volume EM
Flow Cytometry
- Cell Sorting – Beckman Coutler Astrios, a 4 laser 20+ detector system with 6 way, multiwell plate sorting capabilities, Becton Dickinson FACSDiscover S8 imaging cell sorter, plus a Beckman Coulter CytoFLEX SRT for user friendly cell sorting.
- Flow Cytometry Analysers – Beckman Coulter 3x CytoFLEX (2x 6 laser 21 colour LX systems and 1x 4 laser 11 colour S options), BD FACSDiscover S8, BD Fortessa X20. All with multiwell plate loading options.
Applications
Keywords - Biological: Cell Biology | Neurobiology | Developmental Biology | Immunobiology | Plant Biology | Zebrafish | Drosophila | Microbiology | Bio-materials | Materials
Keywords - Material: Material | reflection | fibres | surface | 3D | luminescence
Sample Preparation
Keywords - Biological: Resin embedding | Ultrathin sectioning | Cryosectioning | Immunolabelling | Plunge Freezing | Freeze substitution | Critical Point Drying | Freeze fracture | Metal coating | Negative stain | Photoconversion | Correlative Light and EM | Immunofluorescence | Cryostat sectioning | Vibrating microtome | H&E staining | Live Cell
Users normally prepare their own samples for light microscopy with service provision for resin embedding and sectioning for LM and EM, however all options are available.
Data Analysis
Keywords - Software: ImageJ | FIJI | iQ | ImagePro | Arivis | TissueGnostics | HistoQuest | TissueQuest | FCS Express | FlowJo
Shared Access
The Bioscience Technology Facility is an open-access facility open to internal as well as external commercial & external academic. The microscopes are charged at an hourly rate for DIY work with additional staff costs for full service work. Quotes available for full service work and research contracts where necessary. No extra fee is charged for training.
Funding
The facility works on a full cost recovery basis.