Expertise
Imaging Platforms
Keywords - Light Microscopy: Confocal microscopy | Superresolution microscopy | Widefield microscopy | TIRF | Live cell imaging | Multi-photon microscopy | PALM | STORM | TCSPC-FLIM | Structured Illumination Microscopy
Confocal microscopes:
- Leica SP5 upright: MP laser, TCSPC-FLIM, motorized stage, HyD detectors, quad-NDD detectors
- Leica SP5 invert: MP laser, TCSPC-FLIM, motorized stage, incubation chamber
- Leica SP8 invert with normal and resonant scanner, 2 HYD detectors, DIC optics, motorized stage and incubation chamber
- Leica Stellaris 5 DLS confocal and Light sheet microscope
- Leica Stellaris 8 invert with incubation chamber, White Light Laser and Lightning deconvolution
- Leica Stellaris 8 STED, FALCON FLIM, FCCS invert with incubation chamber
- Zeiss LSM780 invert: motorized stage, spectral unmixing, GaAsP detectors, incubation chamber
- Zeiss Axio Observer 7 in CL2 lab
- Zeiss Cell Discoverer 7 in CL2 lab
Widefield microscopes:
- Zeiss Axio Observer inverted microscope, Colibri LED light sources, Hamamatsu Flash 4.
- Zeiss AxioObserver (Zen, Hamamatsu Flash4 & colour camera): Lumencore SpectraX LED, incubation chamber
- Zeiss AxioObserver (ZenHamamatsu Flash4 & colour camera): Zeiss Colibri LED, incubation chamber
- Zeiss AxioVert South Ken (HCImage, EM-CCD), Xenon lamp, incubation chamber
- Nikon High Content Imaging System
Super-Resolution:
- Zeiss Elyra PS-1 (dual-EM-CCD Andor iXon PCO Edge sCMOS) for PALM, STORM and SIM
Applications
Keywords - Biological: Cell Biology | Developmental Biology | Plant Biology | Zebrafish | Drosophila | Microbiology | Bio-materials
Intravital Microscopy
Confocal microscopy
FRAP
FLIM/FRET
Live Imaging, long-term imaging
Calcium Imaging
Histology (colour-camera)
Data Analysis
Keywords - Software: ImageJ | IMARIS | Fiji | Huygens | Volocity | Icy | Zen
We also provide access to software and expertise in image data analysis using commercial (Huygens, Volocity, Definiens, Zen) and open-source softwares (e.g Icy, Fiji) on local analysis computers or powerful servers.
Shared Access Overview
The facility is open to all college staff and students.
Usage for people from outside the college is possible (subject to availability). If interested, please get in contact.
Usage of any microscope in the facility is only allowed after personal training (see training page for more details). First-time users please get in contact with facility staff to arrange the training.
Funding Overview
The Facility for Imaging by Light Microscopy (FILM) at Imperial College London was established 10 years ago through a variety of sources (research councils, College, etc).
FILM operates under a cost-recovery model and is part supported by funding from the Wellcome Trust (grant 104931/Z/14/Z) and BBSRC (grant BB/L015129/1).