Expertise
Imaging Platforms
Keywords - Light Microscopy: Confocal microscopy | Superresolution microscopy | Widefield microscopy | TIRF | Live cell imaging | Multi-photon microscopy | FCS/FCCS | Atomic Force Microscopy | Light-sheet microscopy (SPIM) | Time-domain FLIM | high-content imaging
- Microscope 1: Zeiss 880 multiphoton confocal microscope
- Microscope 2: Light Sheet Z.1 (Zeiss)
- Microscope 3: Zeiss Elyra7 super-resolution microscope for latticeSIM and SMLM
- Microscope 4: Zeiss LSM780 equipped with a PicoQuant FLIM system for FLIM, phosphorescence FLIM, FLIM-FRET, FCS, RICS and FCCS,
- Microscope 5: Fully motorised inverted Wide-field Zeiss Axio ObserverZ1, equipped with - Andor iXon ULTRA High speed EMCCD camera for SOFI, and Micro-injection station (Narishige).
- Microscope 6: Zeiss 880 BioAFM: hybrid confocal equipped with a JPK AFM (Nanowizard 3) and Zeiss TIRF
- Microscope 7: Histology workstation - Zeiss Axiovert microscope fitted with a Leica DFC450 C
- Microscope 8: ANDOR Dragonfly spinning disk confocal with SRRF and TIRF
- Microscope 9: Zeiss LSM900 confocal microscope with Airyscan2 (4Y)
- Microscope10: Zeiss LSM800 confocal microscope with Airyscan
- Microscope11: 3i Marianas spinning disk confocal microscope with FRET, FRAP and TIRF
- Microscope 12: Leica SP5 confocal microscope (upright)
- Microscope 13 and 14: Sartorius Incucyte S3 high throughput, live cell imaging system
- Microscope 15: Zeiss CellDiscoverer7 with LSM900 and Airyscan2
Applications
KEYWORDS – BIOLOGICAL: CELL BIOLOGY | NEUROBIOLOGY | DEVELOPMENTAL BIOLOGY | IMMUNOBIOLOGY | PLANT BIOLOGY | ZEBRAFISH | C.ELEGANS | DROSOPHILA | MICROBIOLOGY | BIO-MATERIALS | HISTOLOGY/PATHOLOGY | FOOD
KEYWORDS - PHYSICAL SCIENCES: BIOENGINEERING AND BIOMATERIALS |NANOMATERIALS (NANOPARTICLES, 2D NANOMATERIALS, NANOWIRES AND NANOTUBES) | PHARMACOLOGY
As a Liverpool Shared Research Facility on campus, the CCI has been designed to cater for a wide variety of applications, for academy and industry alike. The Liverpool Shared Research Facilities is an organisation within the University of Liverpool Faculty of Health and Life Sciences that promotes access to enabling technology and the academic expertise that supports it.
Sample Preparation
Keywords - Biological: Cryosectioning | Immunolabelling | Plunge Freezing
We are happy to help users to find the best way to prepare their own samples. We can accommodate a variety of samples, ranging from simple slides to multi-well plates and samples embedded in 3D matrices for the light sheet microscope.
Data Analysis
KEYWORDS - SOFTWARE: IMAGEJ | FIJI | IMARIS | MATLAB | Knime Analytics platform | Ilastik | MICROMANAGER | ZEISS ZEN Blue/Black | OMERO
KEYWORDS - HARDWARE: 5 WORKSTATIONS / UPGRADED DATA STORAGE: SERVER 4U DUAL SOCKET INTEL -24 BAYS SAS/SATA, 500TB WITH MIRROR FOR SELECTED DATA, OS RED HAT, HOSTING THE OMERO SOFTWARE (VA TECHNOLOGIES)
Modern biological imaging produces vast amounts of data. At the CCI we have the infrastructure and expertise to be able to support users in everything from short-term storage of a few gigabytes to the long-term storage of terabytes. All of which can be made accessible on or off-site.
We have a suite of dedicated workstations, running a range of software packages (such as Fiji, Matlab and Imaris) that can be used for image analysis and processing, and a full-time Image Analyst.
The Liverpool CCI is proud to have adopted OMERO to facilitate records management and storage. This server-based system greatly facilitates the storage, access (public or private) and archival of imaging data.
Shared Access
The Centre for Cell Imaging was established as a Shared Research Facility (SRF) in May 2011. The CCI is a part of the Liverpool Shared Research Facilities, an evolution of the former Technology Directorate, that encompasses 17 different research facilities across the University (including bioimaging, bioresources and multi-omics) and aims to deliver flexible access to world leading research expertise and equipment.
Funding
The CCI is under the umbrella of the Liverpool Shared Research Facilities. Amongst the last grants, MRC (clinical infrastructures - Next generation microscopy), BBSRC (Alert 13 and Alert 14).