Expertise
Imaging Platform
Keyword - Light Microscopy; Superresolution microscopy | Live cell imaging | Confocal microscopy | Widefield microscopy | TIRF | STORM | Structured Illumination Microscopy
Other: Slide scanning, Quantitative phase imaging, High content screening
Imaging Platform overview:
Confocal microscopy:
- Zeiss LSM 980 Airyscan 2 with environmental chamber
- Olympus FV1000 CLSM with environmental chamber
- Nikon C1Si CLSM
- VisiTech Infinity 3 HAWK 2D array scanner with environmental chamber
Wide field system:
- Zeiss Elyra 7 Lattice SIM2 with TIRF, SMLM, environmental chamber
- 2 Nikon Ti-E wide field systems with environmental chamber
- Olympus IX81 - Inscoper with environmental chamber
- Upright Olympus BX61
Slide scanners:
- Akoya PhenoImager (Vectra Polaris)
- Akoya Phenocycler-Fusion
Quantitative Phase Imaging (label free imaging):
- Phasefocus LiveCyte 2
Applications
Keywords - Biological: Cell Biology | Developmental Biology | Plant Biology | C.elegans | Microbiology | Histology/Pathology | Neurobiology | Immunobiology | Zebrafish | Drosophila | Bio-materials
Data Analysis
Keywords - Software: ImageJ | Imaris | Huygens | Fiji
Other: InForm | VisioPharm | Arivis | NIS-Elements | ScanR-Analysis | ZEN-Blue | LiveCyte analysis
Shared Access
The AIF is an open access facility within the University of Leicester Core Biotechnology Services. We welcome academic and commercial projects from outside the University. Please contact me for further details and access charges.
Funding Overview
The first microscopes were funded in 2001 by grants from the BBSRC and Wellcome Trust. In 2006 the AIF was established and funding for additional microscope systems obtained from the Wolfson Foundation. Several additional systems and upgrades were purchased via CIF funding (2009) and funding from the University Research Equipment and Infrastructure Fund (2012, 2013, 2015, 2018, 2019). The Zeiss LSM980 Airyscan2 was funded via BBSRC ALERT2018, the Akoya PhenoCycler Fusion via MRC mid-range equipment call 2023 and the Zeiss Elyra 7 Lattice SIM2 was funded through BBSRC ALERT22 and installed end 2023.
The maintenance of the facility is partly funded by usage charges.